Trang: 11
Tập 30, số 9 2020
Detecting SARS-CoV-2 by Realtime RT-PCR in clinical samples collected in the North of Vietnam
Tác giả: Hoang Vu Mai Phuong, Ung Thi Hong Trang, Nguyen Vu Son, Le Thi Thanh, Nguyen Le Khanh
Hang, Vuong Duc Cuong, Tran Thi Thu Huong, Pham Thi Hien, Nguyen Phuong Anh, Nguyen
Co Thach, Le Thi Quynh Mai
Tóm tắt:
From January to August 2020, Northern Viet Nam faced a COVID-19 outbreak, up to September 2020, there were
1122 confrmed cases of SARS-CoV-2, of which 465 cases were imported from Europe, America and Asia, 657 cases
were identifed domestically. A total of 30,686 samples were collected during the SARS-CoV-2 outbreak in Northern
Viet Nam and examined by Real-time RT-PCR using primers and probe from Charite - Berlin protocol. This study
showed the initial results of SARS-CoV-2 detection and RNA quantitative in positive samples. The positive rate was
0.8%, ranging from 0.4 to 3.5% according to collection sites. Out of 251 positive samples, the mean Ct value was
28 (IQR: 22.3-32; range 14 - 38). The positive samples had a Ct value below 30 was 68.5%, there was no signifcant
difference between the Ct value of the group ≤ 30 and > 30. The mean of the RNA copies/µl was 8.4.107, (IQR: 2.29.106
- 1.83.109 RNA copies/µl, range: 1.95.103 – 4.95.1011). In the group of imported COVID-19 cases, the rate of virus
at low level was 29%, an average was 56% and at high level was 15%. In the community groups, the viral load data
showed that the average rate at low, intermediate and high level were 20%, 63% and 17% respectively. The proportion
of high-level viral load may raise an alert to start the quarantine process to reduce the transmission of SARS-CoV-2.
Summary:
Từ khóa:
SARS-CoV-2; real-time RT-PCR; North of Viet Nam
File nội dung:
o200911.pdf
Tải file:
